The Effect of Saponin on the Na+ - K+ Activated ATPase of Cattle Lens Epithelial Cell.
- Author:
We Gon CHANG
;
Kwang Lim KOO
;
Byung Nam KANG
- Publication Type:Original Article
- Keywords:
Cataract;
Inorganic phosphate;
Lens epithelium Na+ - K+ ATPase;
Saponin
- MeSH:
Adenosine Triphosphatases*;
Animals;
Calcium;
Cataract;
Cattle*;
Epithelial Cells*;
Epithelium;
Membranes;
Ouabain;
Potassium;
Saponins*;
Sodium
- From:Journal of the Korean Ophthalmological Society
1992;33(1):60-67
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effect of saponin on the Na+ - K+ ATPase of anterior capsule and epithelium of cattle lens has been investigated. The experiment also designed to determine the cation of saponin on the Na+ - K+ ATPase activity in anterior capsule and epithelium of cattle lens. The following results are observed. 1. The Na+ - K+ ATPase activity of cattle lens epithelial cell membrane is inhibited by low concentration of saponin, but increased by high concentration. The strongest activity showed at the dose of 50mg% saponin concentration. 2. The activating effect of saponin on the cattle epithelial cell membrane Na+ - K+ ATPase activity is inhibited by ouabain, but the stimulation of the Mg++ ATPase by high concentration of saponin is not inhibited by ouabain. 3. The activity ratio of Na+ - K+ ATPase by high concentration of saponin is increased by raising the sodium concentration but decreased by raising the potassium concentration. 4. The Na+ - K+ ATPase activity is increased by small amount of calcium but completely inhibited by over 5mM concentration of calcium.
