Ginseng Total Saponin Improves Podocyte Hyperpermeability Induced by High Glucose and Advanced Glycosylation Endproducts.
10.3346/jkms.2011.26.10.1316
- Author:
Tae Sun HA
1
;
Ji Young CHOI
;
Hye Young PARK
;
Jin Seok LEE
Author Information
1. Department of Pediatrics, College of Medicine, Chungbuk National University, Cheongju, Korea. tsha@chungbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Ginseng Total Saponin;
Podocytes;
ZO-1;
Advanced Glycosylation Endproducts;
Diabetic nephropathies
- MeSH:
Actin Cytoskeleton/metabolism;
Animals;
Cell Line;
Diabetic Nephropathies/physiopathology;
Glomerular Filtration Rate;
Glucose/*pharmacology;
Glycosylation End Products, Advanced/*pharmacology;
Hyperglycemia/physiopathology;
Membrane Proteins/*metabolism;
Mice;
*Panax;
Permeability/drug effects;
Phosphoproteins/*metabolism;
Plant Preparations/*pharmacology;
Podocytes/drug effects/pathology/physiology;
Saponins/*pharmacology;
beta Catenin/metabolism
- From:Journal of Korean Medical Science
2011;26(10):1316-1321
- CountryRepublic of Korea
- Language:English
-
Abstract:
Early diabetic nephropathy is characterized by glomerular hyperpermeability as a result of impaired glomerular filtration structure caused by hyperglycemia, glycated proteins or irreversible advanced glycosylation endproducts (AGE). To investigate the effect of ginseng total saponin (GTS) on the pathologic changes of podocyte ZO (zonula occludens)-1 protein and podocyte permeability induced by diabetic conditions, we cultured mouse podocytes under: 1) normal glucose (5 mM, = control); 2) high glucose (HG, 30 mM); 3) AGE-added; or 4) HG plus AGE-added conditions and treated with GTS. HG and AGE increased the dextran filtration of monolayered podocytes at early stage (2-8 hr) in permeability assay. In confocal imaging, ZO-1 colocalized with actin filaments and beta-catenin at cell contact areas, forming intercellular filtration gaps. However, these diabetic conditions suppressed ZO-1 immunostainings and disrupted the linearity of ZO-1. In Western blotting, diabetic conditions also decreased cellular ZO-1 protein levels at 6 hr and 24 hr. GTS improved such quantitative and qualitative changes. These findings imply that HG and AGE have an influence on the redistribution and amount of ZO-1 protein of podocytes thereby causing hyperpermeability at early stage, which can be reversed by GTS.
