Activation Of H-Ras Oncogene In Rat Salivary Gland Tumors Induced By Dmba And Irradiation.
- Author:
Key Soon HU
1
;
Jong Whan CHOI
;
Soon Chul CHOI
;
Tae Won PARK
;
Dong Soo YOU
Author Information
1. Department of Oral & Maxillofacial Radiology, Dental Research Institute, College of Dentistry, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
9,10-Dimethyl-1,2-benzanthracene*;
Animals;
Cell Line, Tumor;
Codon;
DNA;
Genes, ras*;
Genome;
Humans;
NIH 3T3 Cells;
Oncogenes;
Point Mutation;
Rats*;
Salivary Glands*
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1998;28(1):245-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutaions has important prognostic implication in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA-implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the following results were concluded : 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/ DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/ DNA in the experimental group with the DMBA-implantation/irradiation according to results of trasfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9%(5/36) in the experimental group with the DMBA implantation, 15.4%(6/39) in the experimental group with the DMBA-implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6%(2/36) and 8.3%(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7%(3/39) in the experimental group with the DMBA-implantation/irradiation.
