A Study On The Radiosensitivity And Chemosensitivity Of Kb Cell Line In Vitro.
- Author:
Sung Woo HONG
1
;
Eun Suk CHOI
;
Kwang Joon KOH
Author Information
1. Department of Oral & Maxillofacial Radiology, College of Dentistry, Chonbuk National University, Korea.
- Publication Type:In Vitro ; Original Article
- MeSH:
Antineoplastic Agents;
Bleomycin;
Carcinoma, Squamous Cell;
Cisplatin;
Drug Therapy;
Humans;
KB Cells*;
Radiation Tolerance*;
Radiotherapy
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1998;28(1):271-283
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. For this study, cell surviving curves were obtained for human squamous cell carcinoma KB cell line after radiation exposure and/or administration of antitumor drugs. 2, 4, 6, 8 10Gy were irradiated at a dose rate of 210Gy/min using 60Co Irradiator ALDORADO 8. After irradiation, KB cell lines(3*104cells/ml) were exposed to 2 /ml of bleomycin ir cisplatin for 1 hour. The viable cells were determined by MTT assay for each radiation dose and/or each drug at the 4th day. And they were compared to control values. The obtained results were as follows ; 1. The slope of the surviving curve after irradiation of 2, 4, 6, 8, 10Gy on KB cell line was relatively steep. 2. There was no significant difference between ths cytotoxicity of bleomycin compared to control group. But, there was significant difference between the cytotoxicity of cisplatin compared to control group. And the cytotoxicity of cisplatin was greater than that of bleomycin on KB cell line. 3. There were dignificant differences of surviving fractions after irradiation of 2Gy and 10Gy with 2 /ml of bleomycin compared with the groups of irradiation only on KB cell line. 4. There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with 2 /ml of cisplantin compared with the groups of irradiation only on KB cell line. 5, There was significant difference of surviving fraction between groups after irradiation of 10Gy with 2 /ml of bleomycin and cisplatin.
