Hypermethylation of Tumor-related Genes in Genitourinary Cancer Cell Lines.
10.3346/jkms.2001.16.6.756
- Author:
Woon Bok CHUNG
1
;
Su Hyung HONG
;
Jin A KIM
;
Yoon Kyung SOHN
;
Bup Wan KIM
;
Jung Wan KIM
Author Information
1. Department of Dental Microbiology, College of Dentistry, Kyungpook National University, Taegu, Korea. jwkim@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Urogenital Neoplasms;
Genitourinary cancer cell lines;
HMLH1;
GSTP1;
E-cadherin;
VHL;
MGMT;
Hypermethylation;
Methylation-specific PCR
- MeSH:
Bladder Neoplasms/genetics;
Cadherins/genetics;
*DNA Methylation;
DNA Primers;
Genetic Screening/methods;
Glutathione Transferase/genetics;
Human;
Isoenzymes/genetics;
Kidney Neoplasms/genetics;
Ligases/genetics;
Male;
Neoplasm Proteins/genetics;
O(6)-Methylguanine-DNA Methyltransferase/genetics;
Polymerase Chain Reaction;
Prostatic Neoplasms/genetics;
Tumor Cells, Cultured;
Urogenital Neoplasms/*genetics
- From:Journal of Korean Medical Science
2001;16(6):756-761
- CountryRepublic of Korea
- Language:English
-
Abstract:
Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines.
