Development and Evaluation of Acellular Dermal Matrix Originated from Transgenic Porcine Skin for Primates Skin Graft.
- Author:
Kyo Won LEE
1
;
Gyu Seong CHOI
;
Min Jung KIM
;
Ji Seon LEE
;
Hyeon YOON
;
Wook CHUN
Author Information
1. Department of Surgery, Division of Transplantation, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Case Report
- Keywords:
Transgenic pig;
Acellular dermal matrix;
Xeno-skin transplantation
- MeSH:
Acellular Dermis*;
Animal Care Committees;
Biopsy;
Burns;
Contracture;
Haplorhini;
Humans;
Macaca fascicularis;
Primates*;
Skin Transplantation;
Skin*;
Subcutaneous Tissue;
Swine;
Transplants*;
Wounds and Injuries
- From:Journal of Korean Burn Society
2016;19(2):82-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to compare the healing quality of the allogenic acellular dermal matix (ADM) and xenogenic ADM combined with autologous split thicknessskin graft. Xenogenic ADM was obtained from two wild type pigs. Allogenic ADM was obtained from cynomolgus monkeys. ADM was stored with cryo-preservation. Full-thickness skin wounds were made on the back of two cynomolgus monkeys. In one monkey, wounds were covered by allogenic ADM combined with autologous split thickness skin graft (STSG) or autologous STSGonly. In another monkey, wounds were covered by xenogenic ADM combined with autologous skin graft or autologous skin graft only. Skin healing process was observed during 2 weeks and skin biopsies were performed on 3 months after skin transplantation. We obtained IACUC approval (ORIENT-IACUC-16053). Skin on the xenogenic ADM was necrotized 1 week after skin transplantation. Possibly due to the thickness of ADM, which block the blood supply from the subcutaneous tissue to the autologous skin graft. Skin biopsy revealed that less fibrotic change of the skin on the ADM compared with the skin without ADM. Xenogenic ADM can be used in high degree burn patients who can suffered from contracture after healing since it can reduce fibrotic change.
