Detection of p53 Gene Mutations in Bladder Cancer using PCR-SSCP Analysis and Immunohistochemistry.
- Author:
Kang Hyun LEE
1
;
Hyun Jung KIM
;
Ho Seong CHOE
;
Hi Joong AHN
;
Ja June JANG
;
Seok Il HONG
Author Information
1. Department of Urology, Korea Cancer Center Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bladder cancer;
p53 gene;
PCR-SSCP
- MeSH:
DNA;
Exons;
Genes, p53*;
Immunohistochemistry*;
Mucous Membrane;
Point Mutation;
Polymerase Chain Reaction;
Prognosis;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1994;35(8):827-831
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We carried out an immunohistochemical study and DNA analysis of 14 surgical specimens of primary bladder carcinoma and paired sample of normal bladder mucosa to evaluate p53 over expression, point mutation by polymerase chain reaction single-strand conformation polymorphism analysis(PCR-SSCP). The immunohistochemical study demonstrated immunoreactivity for p53 protein in ten cases out of 14(71%) with monoclonal antibody (NCL-p53-DO-7, Novocastra Lab. UK). Mobility shifts were detected by PCR-SSCP in p53 gene exons 4-8 in six cases out of 14(42.9%). p53 gene mutations were observed in invasive cases(85.7%) and superficial cases (50%) by immunohistochemistry. Correlation was not observed between the presence of p53 gene mutations and the histological differentiation and invasiveness of the cancer. Considering frequent p53 alterations in invasive cases, it will be necessary to reevaluate p53 alterations in bladder cancer and the correlation to the prognosis in bladder cancer.
