Preparation and Characterization of SDF-1alpha Recombinant Adenovirus for ex vivo Expansion of Hematopoietic Stem Cells.
- Author:
Eun Kyoung JOO
1
;
Deog Yeon JO
;
Joung Hyuck JOO
;
Pyung Keun MYUNG
;
Younghee LEE
Author Information
1. Department of Biochemistry, Biotechnology Research Institute, College of Natural Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea. yhl4177@chungbuk.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
SDF-1;
Adenovirus;
ex vivo expansion;
HUVEC
- MeSH:
Adenoviridae*;
Adult Stem Cells;
Blood Cells;
Chemokine CXCL12*;
Enzyme-Linked Immunosorbent Assay;
Granulocyte-Macrophage Colony-Stimulating Factor;
Hematopoiesis;
Hematopoietic Stem Cells*;
Human Umbilical Vein Endothelial Cells;
Humans;
Research Personnel;
Stem Cell Factor;
Stem Cells;
Thrombopoietin
- From:Journal of Bacteriology and Virology
2005;35(1):39-47
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Stromal cell-derived factor 1 (SDF-1/CXCL12) is a multifunctional cytokine implicated in normal hematopoiesis. We previously reported that SDF-1alpha enhanced the survival of hematopoietic stem and progenitor cells in synergy with other cytokine such as GM-CSF, steel factor, or thrombopoietin. As adult stem cells are very rare, many investigators are trying to expand hematopoietic stem/progenitor cells in vitro. In this study, we constructed an adenoviral vector and produced high titer of recombinant adenoviruses directing robust expression of SDF-1alpha determined by ELISA. We also produced control empty adenoviruses and recombinant LacZ adenoviruses. In order to check the feasibility of SDF-1alpha in ex vivo expansion system, we compared HUVEC cells tranduced by a SDF-1alpha recombinant virus with HUVEC cells transduced by a LacZ recombinant virus in supporting activity of hematopoietic cells, and found that expression of SDF-1alpha in HUVEC cells increased viable blood cell population obtained from the same number of CD34+ cells. The SDF-1alpha recombinant adenovirus seems to be useful for future application in hematopoiesis studies.
