Matrix Metalloproteinase-2 Expression of the Injured Anterior Cruciate Ligament in the Knee and Changes in Relation to Signal Transduction Pathways.
- Author:
Hwa Sung LEE
1
;
Jae Young KWON
;
Young Kyun WOO
Author Information
1. Department of Orthopedics, St. Mary's Hospital, The Catholic University of Korea, Korea. wooyk@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Knee;
Anterior cruciate ligament;
Stretch;
Matrix metalloproteinase;
Signal transduction pathway
- MeSH:
Animals;
Anterior Cruciate Ligament;
Anthracenes;
Carbazoles;
Curcumin;
Flavonoids;
Gelatin;
Knee;
Ligaments;
Matrix Metalloproteinase 2;
Nitriles;
Pyrroles;
Rats;
Signal Transduction;
Sulfones;
Transcription Factor AP-1;
Weights and Measures
- From:Journal of Korean Orthopaedic Research Society
2008;11(2):64-71
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was designed to pursue the basic relationship between MMP-2 and ligament injury via ex vivo stretching of the anterior cruciate ligament (ACL) and its changes by signal transduction pathway. MATERIALS AND METHODS: After femur-ACL-tibia complex was harvested from rats, release of MMP-2 in stretch group and non-stretch group was checked using gelatin zymography. Firstly, authors investigated whether MMP-2 was released from the ligament or not and if so, how long it was released. In addition, the complexes were divided into two groups. In group I, 5 and 12N weights were used to stretch the complex for 10, 30, and 60-minute. In group II, after the ligament complexes were stretched by 15N for 30 minutes, various signal pathway inhibitors such as pertusis toxin, SP600125, PD98059, KT5720, curcumin, and Bay11-7082 were added to their supernatants. MMP-2 activity was evaluated. RESULTS: MMP-2 was immediately released after ligament injury and its activity was increased in proportion to stretching duration and magnitude. As for the signal pathways, inhibition of JNK, ERK, AP-1, and NF-k beta pathways caused MMP-2 expression to be decreased. CONCLUSION: It is considered that the release of MMP-2 plays an important role in remodeling process after ACL injury.
