Enhanced Atrial Natriuretic Peptide and Nitric Oxide System Following the Treatment with Caffeine in Rats.
- Author:
Jeong Hoon HA
1
;
Chung Ho YEUM
;
Soo Wan KIM
;
Nam Ho KIM
;
Ki Chul CHOI
;
Jong Un LEE
Author Information
1. Research Institute of Medical Sciences, Chonnam National University, Korea. julee@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Caffeine;
Atrial natriuretic peptide;
Nitric oxide;
Aquaporin
- MeSH:
Animals;
Aquaporin 2;
Atrial Natriuretic Factor;
Blotting, Western;
Caffeine*;
Diuresis;
Drinking;
Hand;
Humans;
Kidney;
Male;
Natriuresis;
Nitric Oxide*;
Rats*;
Rats, Sprague-Dawley;
RNA, Messenger
- From:Korean Journal of Nephrology
2002;21(1):123-128
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The present study was aimed to examine whether caffeine alters the local regulation of atrial natriuretic peptide(ANP) and nitric oxide(NO) systems in the kidney. METHODS: Male Sprague-Dawley rats were treated with caffeine, consisting of a single oral bolus(0.2%, 1 mL/kg) followed by supplementation in drinking water(0.2%) for 1 day. Rats treated the same without caffeine served as control. The tissue expression of ANP mRNA was determined by reverse transcription-polymerase chain reaction. Tissue levels of nitrite/nitrate were determined. The expression of aquaporin(AQP)-1 and AQP2 proteins was determined by Western blot analysis. RESULTS: Following the treatment with caffeine, the expression of ANP mRNA was increased in the kidney. The renal tissue nitrite/nitrate level was also increased by caffeine-treatment. On the other hand, the expression of AQP1 and AQP2 proteins was not significantly altered. CONCLUSION: The enhanced activities of local ANP and NO systems may in part be causally related with the caffeine-induced natriuresis and diuresis, while AQP channels are not involved.
