The Role of Nuclear Factor-E2-Related Factor 1 in the Oxidative Stress Response in MC3T3-E1 Osteoblastic Cells.
10.3803/EnM.2016.31.2.336
- Author:
So Young PARK
1
;
Sung Hoon KIM
;
Hyun Koo YOON
;
Chang Hoon YIM
;
Sung Kil LIM
Author Information
1. Department of Internal Medicine, Cheil General Hospital & Women's Healthcare Center, Dankook University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
NF-E2-related factor 1;
Oxidative stress;
Osteoblasts
- MeSH:
Antioxidants;
Catalytic Domain;
Cell Line;
Glutathione Peroxidase;
Inflammation;
Metabolism;
Metallothionein;
NF-E2-Related Factor 1;
Osteoblasts*;
Oxidative Stress*;
Reactive Oxygen Species;
RNA Interference;
RNA, Messenger;
RNA, Small Interfering
- From:Endocrinology and Metabolism
2016;31(2):336-342
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Reactive oxygen species (ROS) and antioxidants are associated with maintenance of cellular function and metabolism. Nuclear factor-E2-related factor 1 (NFE2L1, Nrf1) is known to regulate the expression of a number of genes involved in oxidative stress and inflammation. The purpose of this study was to examine the effects of NFE2L1 on the response to oxidative stress in osteoblastic MC3T3-E1 cells. METHODS: The murine calvaria-derived MC3T3-E1 cell line was exposed to lipopolysaccharide (LPS) for oxidative stress induction. NFE2L1 effects were evaluated using small interfering RNA (siRNA) for NFE2L1 mRNA. ROS generation and the levels of known antioxidant enzyme genes were assayed. RESULTS: NFE2L1 expression was significantly increased 2.4-fold compared to the control group at 10 µg/mL LPS in MC3T3-E1 cells (P<0.05). LPS increased formation of intracellular ROS in MC3T3-E1 cells. NFE2L1 knockdown led to an additional increase of ROS (20%) in the group transfected with NFE2L1 siRNA compared with the control group under LPS stimulation (P<0.05). RNA interference of NFE2L1 suppressed the expression of antioxidant genes including metallothionein 2, glutamatecysteine ligase catalytic subunit, and glutathione peroxidase 1 in LPS-treated MC3T3-E1 cells. CONCLUSION: Our results suggest that NFE2L1 may have a distinct role in the regulation of antioxidant enzymes under inflammation-induced oxidative stress in MC3T3-E1 osteoblastic cells.
