Molecular Diagnosis of Cutaneous T Cell Lymphoproliferative Diseases.
- Author:
Ji Young PARK
;
Myung Hoon LEE
;
Eun Kyung KWAK
;
Dong Ja KIM
;
Tae In PARK
;
Han Ik BAE
- Publication Type:Original Article
- Keywords:
Polymerase chain reaction;
Single strand conformational polymorphism;
Heteroduplex;
T-cell receptor;
Cutaneous T-cell lymphoproliferative diseases
- MeSH:
Biopsy;
Diagnosis*;
DNA;
Gene Rearrangement;
Genes, T-Cell Receptor;
Genes, T-Cell Receptor beta;
Heteroduplex Analysis;
Humans;
Jurkat Cells;
Lymphoma, T-Cell;
Lymphomatoid Papulosis;
Lymphoproliferative Disorders;
Mycosis Fungoides;
Paraffin;
Polymerase Chain Reaction;
Receptors, Antigen, T-Cell;
Skin;
T-Lymphocytes
- From:Korean Journal of Pathology
2000;34(11):941-949
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It is often problematic to diagnose T-cell lymphoproliferative disorders of the skin because of the difficulty in establishing clonality in paraffin-embedded tissue. We used polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and heteroduplex analysis in paraffin embedded tissue to detect clonal rearrangement of T-cell receptor gamma (TCRgamma) gene in 17 T-cell lymphoproliferative disorders and 6 atypical lymphoproliferative diseases. We used polymerase chain reaction to detect TCR beta gene rearrangement in 8 of 17 cases which did not show TCRgamma gene rearrangement. Jurkat cell lines were used as monoclonal controls. DNA was extracted from 5 biopsies of T-cell lymphomas, 10 biopsies of mycosis fungoides, 2 biopsies of lymphomatoid papulosis, and 6 biopsies of atypical lymphoproliferative lesions. We detected monoclonality in 5 of 5 T-cell lymphoma cases, 2 of 2 lymphomatoid papulosis cases, 6 of 10 mycosis fungoides cases, and 2 of 6 atypical lymphoproliferative disease cases. We conclude that nonradioactive PCR-SSCP for TCR gene rearrangement analysis is a useful adjunct to routine histological and immunophenotypic methods in the diagnosis of cutaneous T cell lymphoproliferative disorders in paraffin embedded tissue.
