Organotypic slice culture of the hypothalamic paraventricular nucleus of rat.
- Author:
Eun Seong CHO
1
;
So Yeong LEE
;
Jae Yong PARK
;
Seong Geun HONG
;
Pan Dong RYU
Author Information
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords: electrophysiology; green fluorescent protein; immunocytochemistry; organotypic slice culture; paraventricular nucleus
- MeSH: Adenoviridae; Animals; Cell Culture Techniques/*methods; Corticotropin-Releasing Hormone/metabolism; Electrophysiology; Genetic Vectors; Green Fluorescent Proteins/metabolism; Immunohistochemistry; Neurons/*cytology/metabolism; Oxazines; Oxytocin/metabolism; Paraventricular Hypothalamic Nucleus/*anatomy & histology/cytology/metabolism; Rats; Vasopressins/metabolism
- From:Journal of Veterinary Science 2007;8(1):15-20
- CountryRepublic of Korea
- Language:English
- Abstract: Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
