Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE.
- Author:
Eun Kyung SHIN
1
;
Yeon Soo SEO
;
Jeong Hee HAN
;
Tae Wook HAHN
Author Information
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords: Bordetella bronchiseptica; genetic diversity; PFGE; RAPD
- MeSH: Animals; Bordetella bronchiseptica/*genetics; Cluster Analysis; DNA Primers; Electrophoresis, Gel, Pulsed-Field; *Genetic Variation; Korea; Random Amplified Polymorphic DNA Technique; Species Specificity; Swine/*microbiology
- From:Journal of Veterinary Science 2007;8(1):65-73
- CountryRepublic of Korea
- Language:English
- Abstract: The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA-07 which was shared by 32 isolates (71.1%), the pattern produced by OPA-08 was shared by 26 isolates (57.8%), and the pattern produced by OPA-18 was shared by 40 isolates (88.9%). The RAPD patterns of the vaccine strain and the 3 reference strains did not match any of the patterns produced by the field isolates when primers OPA-07 and OPA-08 were used. PFGE using the restriction endonuclease XbaI produced a total of 15 patterns consisting of 4 PFGE types (A, B, B1 and C, differing by > or = 4 bands) and 11 A subtypes (differing by < or = 3 bands). Most of the field isolates exhibited identical type A and B patterns, suggesting that they were related. The vaccine strain and the three reference strains showed different PFGE patterns as compared to the identical type A strains.
