Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos.
- Author:
Kilyoung SONG
1
;
Eunsong LEE
Author Information
- Publication Type:Original Article ; Comparative Study ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords: embryo development; oocyte maturation; parthenogenesis; pig; somatic cell nuclear transfer
- MeSH: Animals; Cysteamine; Embryo Culture Techniques/*veterinary; Embryo, Mammalian/*physiology; Female; Follicular Fluid; Mercaptoethanol; Nuclear Transfer Techniques/*veterinary; Oocytes/*growth & development; Sus scrofa/*physiology; Time Factors
- From:Journal of Veterinary Science 2007;8(1):81-87
- CountryRepublic of Korea
- Language:English
- Abstract: This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or beta-mercaptoethanol (beta-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 micrometer CYS or 100 micrometer beta-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. beta-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos.
