Age - related Changes of T cells,B cells Distribution in C57BL/6 Mice Thymus,Spleen and Popliteal Lymph Node.
10.11637/kjpa.1998.11.2.361
- Author:
Sung Bae CHO
1
;
Won Kyu LEE
;
Dong Hwan SHIN
;
Chung Man HONG
;
Young Buhm HUR
;
Hee Kyung AHN
Author Information
1. Department of Anatomy, College of Medicine, Kyunghee University, Korea.
- Publication Type:Original Article
- Keywords:
Thymus;
Spleen;
Lymph Node;
Mouse;
Age
- MeSH:
Animals;
Avidin;
B-Lymphocytes;
Cell Count;
Immunity, Cellular;
Immunoglobulin M;
Lymph Nodes*;
Mice*;
Parturition;
Spleen;
T-Lymphocytes;
Thymus Gland
- From:Korean Journal of Physical Anthropology
1998;11(2):361-375
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The following experiment was performed to clarify distributional changes of the Ly1, L3T4, Ly2 positive T cells and IgM positive B cells 1, 4, 8, 12 and 40 weeks after birth. Thymus, spleen and lymph node were removed and immunohistochemical staining such as avidin -biotin -peroxidase complex method was used. The results obtained from above epxperiment were as follows; 1. Ly1 positive T cells were decreased after 8 weeks in the thymus and spleen. these were decreased after 40 weeks in the lymph node. 2. There was no difference L3T4 positive T cells in the thymus, but in the spleen decrease of cell number was shown after 40 weeks. 3. Ly2 postive T cells were decreased after 4 weeks in the thymus, after 40 weeks in the spleen. There was no change of distribution in the lymph node. 4. There was no difference distribution of IgM positive B cells. The results suggest that the age related decrease of the immunity is caused by decrease of cellular immunity related to T cell depletion.
