Migrating motor complex changes after side-to-side ileal bypass in mouse ileum ex-vivo: mechanism underlying the blind loop syndrome?.
10.4174/jkss.2011.80.4.251
- Author:
Suk Bae MOON
1
;
Kyu Joo PARK
;
Jung Sun MOON
;
Eun Kyoung CHOE
;
In Suk SO
;
Sung Eun JUNG
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. kjparkmd@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Myoelectric complex;
Migrating;
Mice;
Ileum;
Jejunoileal bypass
- MeSH:
Animals;
Area Under Curve;
Blind Loop Syndrome;
Female;
Humans;
Ileum;
Jejunoileal Bypass;
Mice;
Myoelectric Complex, Migrating
- From:Journal of the Korean Surgical Society
2011;80(4):251-259
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was intended to investigate the migrating motor complex (MMC) changes after ileal bypass in ex-vivo mouse models. METHODS: Partial (side-to-side) and total bypass (occlusion of proximal part of bypassed loop) were performed on ileums of female Institute of Cancer Research mice. After 2 and 4 weeks, the bypassed segments were harvested and MMCs were recorded at 4 different sites ex-vivo. Amplitude, duration, interval, direction of propagation, and the area under the curve (AUC) of MMCs were measured and compared to those of the controls. RESULTS: In control mice (n = 7), most MMCs propagated aborally (91.1%). After 2 weeks of partial bypass (n = 4), there was a significant decrease in both amplitude and AUC, and orally-propagating MMCs increased significantly (45%, P = 0.002). Bidirectional MMCs (originating in the bypassed loop and propagating in both directions) were also observed (10%). The amplitude of the MMCs remained decreased at 4 weeks after partial bypass (n = 4), and neither the AUC nor the direction of propagation showed significant changes compared to 2 weeks. Similarly, in the total bypass model, both the amplitude and AUC of the MMCs decreased significantly compared to controls. In contrast to partial bypass, 95% of the MMCs within the bypassed loop propagated aborally after 2 weeks (n = 6), which was similar to the control state. After 4 weeks (n = 5), however, MMCs either lost their temporal relationship or completely disappeared. CONCLUSION: The changes in propagation direction of the MMCs in the partially bypassed loop may contribute to stagnation of bowel contents and the development of blind loop syndrome.
