miR-379 Inhibits Cell Proliferation, Invasion, and Migration of Vascular Smooth Muscle Cells by Targeting Insulin-Like Factor-1.
10.3349/ymj.2017.58.1.234
- Author:
Kai LI
1
;
Yong WANG
;
Anji ZHANG
;
Baixue LIU
;
Li JIA
Author Information
1. Department of Cardiology, The First Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi Province, China. yong_wang0756@sina.com
- Publication Type:Original Article
- Keywords:
Vascular smooth muscle cells;
miR-379;
cell proliferation;
invasion;
migration;
IGF-1
- MeSH:
Apoptosis;
Cell Movement/*physiology;
Cell Proliferation/*physiology;
Humans;
Insulin;
Insulin-Like Growth Factor I/*physiology;
MicroRNAs/*physiology;
Muscle, Smooth, Vascular/*cytology;
Proto-Oncogene Proteins c-sis/*physiology;
RNA, Messenger/metabolism;
Real-Time Polymerase Chain Reaction;
Sincalide/physiology;
Wound Healing/physiology
- From:Yonsei Medical Journal
2017;58(1):234-240
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: MicroRNAs are small non-coding RNAs that play important roles in vascular smooth muscle cell (VSMC) function. This study investigated the role of miR-379 on proliferation, invasion, and migration of VSMCs and explored underlying mechanisms thereof. MATERIALS AND METHODS: MicroRNA, mRNA, and protein levels were determined by quantitative real-time PCR and western blot. The proliferative, invasive, and migratory abilities of VSMCs were measured by CCK-8, invasion, and wound healing assay, respectively. Luciferase reporter assay was used to confirm the target of miR-379. RESULTS: Platelet-derived growth factor-bb was found to promote cell proliferation and suppress miR-379 expression in VSMCs. Functional assays demonstrated that miR-379 inhibited cell proliferation, cell invasion, and migration. Flow cytometry results further showed that miR-379 induced apoptosis in VSMCs. TargetScan analysis and luciferase report assay confirmed that insulin-like growth factor-1 (IGF-1) 3'UTR is a direct target of miR-379, and mRNA and protein levels of miR-379 and IGF-1 were inversely correlated. Rescue experiments showed that enforced expression of IGF-1 sufficiently overcomes the inhibitory effect of miR-379 on cell proliferation, invasion, and migration in VSMCs. CONCLUSION: Our results suggest that miR-379 plays an important role in regulating VSMCs proliferation, invasion, and migration by targeting IGF-1.
