Characterization of Salmonella spp. Clinical Isolates in Gyeongsangbuk-do Province, 2012 to 2013.
- Author:
Oh Geun KWEON
1
;
Jin Seok KIM
;
Gou Ok KIM
;
Chang Il LEE
;
Kwang Hyeon JEONG
;
Junyoung KIM
Author Information
- Publication Type:Original Article
- Keywords: Drug resistance; Extended-spectrum beta-lactamase; Nalidixic acid; Pulsed-Field Gel Electrophoresis; Salmonella
- MeSH: Ampicillin; beta-Lactamases; Cephalosporins; Chloramphenicol Resistance; Drug Resistance; Drug Resistance, Microbial; Electrophoresis, Gel, Pulsed-Field; Fluoroquinolones; Gyeongsangbuk-do; Nalidixic Acid; Plasmids; Prevalence; Salmonella Infections; Salmonella*
- From:Annals of Clinical Microbiology 2014;17(2):50-57
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Extended-spectrum cephalosporins and fluoroquinolones are important antimicrobials for treating invasive salmonellosis, and emerging resistance to these antimicrobials is of paramount concern. METHODS: A total of 30 Salmonella spp. clinical isolates recovered in Gyeongsangbuk-do from 2012 to 2013 were characterized using antibiotic resistance profiles and pulsed-field gel electrophoresis (PFGE). RESULTS: A high prevalence of multidrug-resistant isolates, mainly showing an ampicillin, nalidixic acid, chloramphenicol resistance pattern, was observed. Four extended-spectrum beta-lactamase (ESBL)-producing isolates (3 CTX-M-15 isolates and 1 CTX-M-27 isolate) were found. The bla(CTX-M-27) gene was carried by an IncF conjugative plasmid in the S. Infantis isolate. The bla(CTX-M-15) gene were carried by an IncF (2 isolates) or IncHI2 (1 isolate) conjugative plasmid in S. Enteritidis. In addition, a single mutation of GyrA, Ser83Thr (1 isolates), Asp87Tyr (9 isolates), Asp87Gly (4 isolates), and Asp87Leu (3 isolates), was detected in nalidixic acid-resistant Salmonella spp. isolates. XbaI PFGE analysis of all isolates revealed more than 19 different pulsotypes. The most common S. Enteritidis PFGE pattern (SEGX01.003) was associated with a larger number of cases of invasive salmonellosis than all other patterns. CONCLUSION: The information from our study can assist in source attribution, outbreak investigations, and tailoring of interventions to maximize disease prevention.
