Effects of MK-801 (dizocilpine) on Brain Cell Membrane Function and Energy Metabolism in Experimental Escherichia coli Meningitis in the Newborn Piglet.
10.3346/jkms.2003.18.2.236
- Author:
Sun Young KO
1
;
Jae Won SHIM
;
Sung Shin KIM
;
Mi Jung KIM
;
Yun Sil CHANG
;
Won Soon PARK
;
Son Moon SHIN
;
Mun Hyang LEE
Author Information
1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. wspark@smc.samsung.co.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Meningitis, Bacterial;
Excitatory Amino Acid Antagonists;
Energy Metabolism;
Brain;
Animals, Newborn
- MeSH:
Animals;
Animals, Newborn;
Blood Glucose/metabolism;
Brain/cytology;
Brain/drug effects*;
Brain/metabolism;
Cell Membrane/drug effects*;
Cell Membrane/metabolism;
Cerebral Cortex/metabolism;
Dizocilpine Maleate/pharmacology*;
Energy Metabolism*;
Excitatory Amino Acid Antagonists/pharmacology;
Glutamic Acid/cerebrospinal fluid;
Lactic Acid/blood;
Leukocytes/metabolism;
Meningitis, Escherichia coli/drug therapy;
Meningitis, Escherichia coli/metabolism*;
Neurons/drug effects*;
Neurons/metabolism;
Neuroprotective Agents/pharmacology*;
Random Allocation;
Swine;
Tumor Necrosis Factor/cerebrospinal fluid
- From:Journal of Korean Medical Science
2003;18(2):236-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
We evaluated the efficacy of non-competitive N-methyl-D-aspartate receptor antagonist MK-801 (dizocilpine) as an adjuvant therapy in experimental neonal bacterial meningitis. Meningitis was induced by injecting 10(6) colony forming units of Escherichia coli into the cisterna magna. MK-801 3 mg/kg was given as a bolus intravenous injection, 30 min before the induction of meningitis. MK-801 did not down-modulate the inflammatory parameters, such as increased intracranial pressure, cerebrospinal fluid (CSF) leukocytosis, increased lactate and TNF-alpha levels in the CSF, and hypoglycorrhachia observed in the meningitis group. MK-801 did not significantly attenuate the elevated glutamate concentration in the CSF. However, MK-801 showed some neuroprotective effects as evidenced by significant attenuation of cerebral lipid peroxidation products (conjugated dienes) and increase of brain high-energy phosphate compounds (ATP and PCr). Improvement in cerebral cortical cell membrane Na+, K+ -ATPase activity did not reach a statistical significance. These results suggest that MK-801 was effective in ameliorating brain injury in neonatal bacterial meningitis, although it failed to attenuate the inflammatory responses.
