Blood-Brain Barrier Experiments with Clinical Magnetic Resonance Imaging and an Immunohistochemical Study.
10.3340/jkns.2010.47.3.203
- Author:
Jun Woo PARK
1
;
Hak Jin KIM
;
Geun Sung SONG
;
Hyung Soo HAN
Author Information
1. Department of Neurosurgery, College of Medicine & Medical Research Institute, Pusan National University, Yangsan, Korea. gnsong@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Animal;
Blood-brain barrier;
Magnetic resonance image;
Immunohistochemical study;
Vasogenic edema;
Vascular permeability
- MeSH:
Animals;
Antigens, Surface;
Blood-Brain Barrier;
Brain;
Capillary Permeability;
Carotid Arteries;
Cerebral Infarction;
Dextrans;
Edema;
Femoral Artery;
Fluorescein;
Humans;
Isothiocyanates;
Magnetic Resonance Imaging;
Magnetic Resonance Spectroscopy;
Magnetics;
Magnets;
Male;
Oleic Acid;
Perfusion;
Rats;
Rats, Sprague-Dawley;
Tetrazolium Salts;
Triolein
- From:Journal of Korean Neurosurgical Society
2010;47(3):203-209
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The purpose of study was to evaluate the feasibility of brain magnetic resonance (MR) images of the rat obtained using a 1.5T MR machine in several blood-brain barrier (BBB) experiments. METHODS: Male Sprague-Dawley rats were used. MR images were obtained using a clinical 1.5T MR machine. A microcatheter was introduced via the femoral artery to the carotid artery. Normal saline (group 1, n = 4), clotted autologous blood (group 2, n = 4), triolein emulsion (group 3, n = 4), and oleic acid emulsion (group 4, n = 4) were infused into the carotid artery through a microcatheter. Conventional and diffusion-weighted images, the apparent coefficient map, perfusion-weighted images, and contrast-enhanced MR images were obtained. Brain tissue was obtained and triphenyltetrazolium chloride (TTC) staining was performed in group 2. Fluorescein isothiocyanate (FITC)-labeled dextran images and endothelial barrier antigen (EBA) studies were performed in group 4. RESULTS: The MR images in group 1 were of good quality. The MR images in group 2 revealed typical findings of acute cerebral infarction. Perfusion defects were noted on the perfusion-weighted images. The MR images in group 3 showed vasogenic edema and contrast enhancement, representing vascular damage. The rats in group 4 had vasogenic edema on the MR images and leakage of dextran on the FITC-labeled dextran image, representing increased vascular permeability. The immune reaction was decreased on the EBA study. CONCLUSION: Clinical 1.5T MR images using a rat depicted many informative results in the present study. These results can be used in further researches of the BBB using combined clinical MR machines and immunohistochemical examinations.
