Adequate Microsatellite Markers for 1p/19q Loss of Heterozygosity of Oligodendroglial Tumors in Korean Patients.
- Author:
Se Hoon KIM
1
;
Hoguen KIM
;
Tai Seung KIM
Author Information
1. Department of Pathology, College of Medicine, Yonsei University, Seoul, Korea. solto@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Oligodendroglioma;
Chromosomes human pair 1;
Chromosomes human pair 19;
Loss of heterozygosity;
Markers microsatellite
- MeSH:
Humans;
Loss of Heterozygosity*;
Microsatellite Repeats*;
Oligodendroglioma;
Paraffin;
Polymerase Chain Reaction;
Prognosis
- From:Korean Journal of Pathology
2005;39(1):23-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It is well known that oligodendrogliomas can be divided into two groups according to the 1p/19q or 1p loss of heterozygosity (LOH) status because oligodendrogliomas with the 1p/19q LOH or the 1p LOH have a better prognosis and chemosensitivity. In this study, we investigated the adequate microsatellite markers for 1p/19q LOH of oligodendroglial tumors in Korean patients. METHODS: We performed PCR that was based on the LOH test with the 1p (D1S508, D1S199, D1S2734, D1S186 & D1S312) and 19q (D19S219, D19S112, D19S412 & D19S596) microsatellite markers; these were the markers that were recommended by other researchers. We performed this PCR on microdissected paraffin embedded tissue blocks of 67 tumors from 56 cases. RESULTS: The PCR based LOH analysis revealed that 3 microsatellite markers (D1S508, D1S2734 & D1S186) of 1p and 2 markers (D19S219 & D19S412) of 19q had higher heterozygosity scores than other markers. In addition, chromosomal LOH status using these selective markers showed a statistically significant difference of prognosis for oligodendroglial tumors. CONCLUSIONS: We can suggest that the microsatellite markers with high heterozygosity scores (D1S508, D1S2734, D1S186, D19S219 and D19S412) would be adequate microsatellite markers for a PCR based LOH test of oligodendroglial tumors in Korean patients.
