Activation of the wnt/β-Catenin Signaling Pathway in Polymyositis, Dermatomyositis and Duchenne Muscular Dystrophy.
10.3988/jcn.2016.12.3.351
- Author:
Fuchen LIU
1
;
Zonglai LIANG
;
Jingwen XU
;
Wei LI
;
Dandan ZHAO
;
Yuying ZHAO
;
Chuanzhu YAN
Author Information
1. Department of Neurology, Qilu Hospital of Shandong University, Jian, China. chuanzhuyan@163.com
- Publication Type:Original Article
- Keywords:
β-catenin;
dermatomyositis;
polymyositis;
duchenne muscular dystrophy;
muscle biopsy
- MeSH:
Blotting, Western;
Cytoplasm;
Dermatomyositis*;
Electrophoretic Mobility Shift Assay;
Embryonic Development;
Female;
Fluorescent Antibody Technique;
Genes, Reporter;
Homeostasis;
Humans;
Luciferases;
Lung Diseases, Interstitial;
Muscular Dystrophy, Duchenne*;
Polymyositis*;
Pregnancy;
Pulmonary Fibrosis;
Regeneration;
Wound Healing
- From:Journal of Clinical Neurology
2016;12(3):351-360
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND PURPOSE: The wnt/β-catenin signaling pathway plays a critical role in embryonic development and adult-tissue homeostasis. Recent investigations implicate the importance of wnt/β-catenin signaling in normal wound healing and its sustained activation being associated with fibrogenesis. We investigated the immunolocalization and activation of wnt/β-catenin in polymyositis (PM), dermatomyositis (DM), and Duchenne muscular dystrophy (DMD). METHODS: Immunofluorescence staining and Western blot analysis of β-catenin were performed in muscle specimens from 6 PM, 8 DM, and 6 DMD subjects. The β-catenin/Tcf4 DNA-binding activity in muscle was studied using an electrophoretic mobility shift assay (EMSA), and serum wnt/β-catenin/Tcf transcriptional activity was measured using a luciferase reporter gene assay. RESULTS: Immunoreactivity for β-catenin was found in the cytoplasm and nuclei of muscle fibers in PM, DM, and DMD. The protein level of β-catenin was elevated, and EMSA analysis confirmed the activation of wnt/β-catenin signaling. The transcriptional activities of β-catenin/Tcf in the circulation were increased in patients with PM, DM, and DMD, especially in those with interstitial lung disease, and these transcriptional activities decreased when PM or DM patients exhibited obvious clinical improvements. CONCLUSIONS: Our findings indicate that wnt/β-catenin signaling is activated in PM, DM, and DMD. Its activation in muscle tissue and the circulation may play a role in modulating muscle regeneration and be at least partly involved in the process of muscle and pulmonary fibrosis.
