Carnosic Acid Inhibits Lipid Accumulation in 3T3-L1 Adipocytes Through Attenuation of Fatty Acid Desaturation.
10.15430/JCP.2015.20.1.41
- Author:
Mi Young PARK
1
;
Mi Kyung SUNG
Author Information
1. Department of Food and Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan, Korea.
- Publication Type:Original Article
- Keywords:
3T3-L1 adipocytes;
Carnosic acid;
Differentiation;
Fatty acid
- MeSH:
Adipocytes*;
Adipogenesis;
Adipose Tissue;
Biomarkers;
Down-Regulation;
Glycerol;
Oxidoreductases;
Peroxisomes;
RNA, Messenger;
Stearoyl-CoA Desaturase;
Sterol Regulatory Element Binding Protein 1;
Triglycerides
- From:Journal of Cancer Prevention
2015;20(1):41-49
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Excess body fat accumulation contributes to the development of metabolic disorders that can cause adverse health effects. Carnosic acid (CA), a major bioactive component of rosemary (Rosemarinus officinalis), has been suggested to possess anti-adipogenic properties. The present study was conducted to elucidate the mechanism underlying the anti-adipogenic effects of CA. METHODS: 3T3-L1 pre-adipocytes were treated with CA (0.1, 1, and 10 muM) from day 0 to day 8 of differentiation. On day 8, biochemical markers of lipid accumulation and the degree of fatty acid desaturation were measured. RESULTS: Oil Red O staining results, triglyceride (TG) accumulation, and glycerol 3-phosphate dehydrogenase activity suggested that CA significantly inhibited lipid accumulation in 3T3-L1 adipocytes. CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-gamma, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-alpha in a dose-dependent manner. Moreover, it decreased the ratio of both C16:1/C16:0 and C18:1/C18:0, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein. CONCLUSIONS: These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.
