Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases.
10.3343/alm.2012.32.5.359
- Author:
Sang Sook HONG
1
;
Kyeongmi KIM
;
Ji Young HUH
;
Bochan JUNG
;
Myung Seo KANG
;
Seong Geun HONG
Author Information
1. Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea. hlseo@cha.ac.kr
- Publication Type:Brief Communication
- Keywords:
Carbapenemase;
Multiplex PCR;
KPC;
GES
- MeSH:
Bacterial Proteins/*genetics/metabolism;
DNA Primers/metabolism;
Databases, Genetic;
Humans;
Klebsiella Infections/microbiology;
Klebsiella pneumoniae/genetics/isolation & purification/metabolism;
*Multiplex Polymerase Chain Reaction;
beta-Lactamases/*genetics/metabolism
- From:Annals of Laboratory Medicine
2012;32(5):359-361
- CountryRepublic of Korea
- Language:English
-
Abstract:
In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-beta-lactamase nonproducers.
