Performance of the BacT Alert 3D System Versus Solid Media for Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis in a Tertiary Hospital in Korea.
10.4046/trd.2016.79.4.282
- Author:
Seoung Cheol KIM
1
;
Bo Young JEON
;
Jin Sook KIM
;
In Hwan CHOI
;
Jiro KIM
;
Jeongim WOO
;
Soojin KIM
;
Hyeong Woo LEE
;
Monoldorova SEZIM
;
Sang Nae CHO
Author Information
1. Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Tuberculosis;
Drug Susceptibility;
Mycobacterium tuberculosis;
Tertiary Care Center;
Korea
- MeSH:
Diagnosis;
Drug Resistance;
Drug Resistance, Microbial;
Extensively Drug-Resistant Tuberculosis;
Humans;
Isoniazid;
Korea*;
Methods;
Mycobacterium tuberculosis*;
Mycobacterium*;
Rifampin;
Sputum;
Tertiary Care Centers*;
Tuberculosis
- From:Tuberculosis and Respiratory Diseases
2016;79(4):282-288
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. METHODS: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. RESULTS: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). CONCLUSION: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.
