Use of Protected Specimen Brush for the Diagnosis of Pulmonary Infection.
- Author:
Jae Myung LEE
;
Dong Kyu KIM
;
Jeong Eun CHOI
;
Dong Hwan KIM
;
Eun Kyung MO
;
Myung Jae PARK
;
Myung Goo LEE
;
In Gyu HYUN
;
Ki Suck JUNG
- Publication Type:Original Article
- Keywords:
Protected specimen brushing;
Pneumonia
- MeSH:
Biopsy;
Bronchoscopes;
Diagnosis*;
Female;
Humans;
Lactic Acid;
Mouth;
Pneumonia;
Sensitivity and Specificity;
Sputum;
Stem Cells
- From:Korean Journal of Medicine
1997;53(2):147-152
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Culture of sputum is apt to be contaminated through oral cavity and proximal airway. Therefore, identification of true etiologic agents by sputum culture is not always reliable. In order to differentiate the pulmonary infection from non-infectious disease and to identify the true etiologic agent of acute pulmonary infection, we used PSB(Protected Specimen Brushing) and evaluated the efficacy of PSB. METHODS: In 168 patients with acute febrile illness with pulmonary infiltrations(male 106, female: 61, mean age: 49.5+/-17.6), we performed PSB via a bronchoscope and compared the results along with blood culture and sputum culture. Protected specimen brush was introduced through biopsy channel of bronchoscope and was rotated within the purulent secretions. Tip of the brush was severed with aseptic technique and was immersed in 1cc of Ringer's lactate solution and vigorously mixed for 1 minute. The specimen was submitted for quantitative culture within 15 minutes and was regarded positive culture if colony forming units were above 10(3)/ml. RESULTS: Using PSB for the diagnosis of pulmonary infection, sensitivity was 71.1% and specificity was 84.296. PSB was helpful in identifing true etiologic agent among several potentially pathogenic organisms. Using PSB for the diagnosis of UAP (ventilator associated pneumonia), sensitivity was 72.4% and specificity was 100%. CONCLUSION: Use of PSB can be a helpful method for the diagnosis of pulmonary infection and identification of its etiologic agents.
