Two A(weak)B Cases with Aw10 Allele Separated by Allele-specific Sequencing.
- Author:
Eun Jeong WON
1
;
Duck CHO
;
Myung Geun SHIN
;
So Yong KWON
;
Nam Sun CHO
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dcho@chonnam.ac.kr
- Publication Type:Case Report
- Keywords:
ABO gene;
Allele separation;
Allele-specific sequencing
- MeSH:
Alleles;
Genotype;
Haplotypes;
Phenotype
- From:Korean Journal of Blood Transfusion
2011;22(1):59-64
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We separated an Aw10 allele by allele-specific sequencing in two Aw10/B101 samples that had the AweakB phenotype. Two samples with the A102/B101 genotype were also tested as a control. The reverse primers using position 930 at exon7 were designed for allele-specific sequencing. The differential positions were a total of 52 points for distinguishing the A-allele from the B-allele. Although overlaps with another haplotype allele that showed a minor chromatographic peak were observed in almost all the points, the specific allele-separation rate was 100% (52/52) by assessing the dominance in the chromatographic peak height. Based on the separation rate in the two cases with Aw10/B101 and the two AB controls, allele-specific sequencing is a convenient and reliable method for the separating the A-allele and B-allele in a clinical laboratory.
