Comparison of the results of quantitative real-time PCR, and AFB stain of tissue and slit skin smear in Hansen's disease.
- Author:
Jong Pill KIM
1
Author Information
1. Institute for Leprosy Research, Korean Hansen Welfare Association, Korea. dr_jpkim@hotmail.com
- Publication Type:Original Article
- Keywords:
slit skin smear;
16S rRNA/RLEP ratio;
viablity
- MeSH:
DNA;
Humans;
Leprosy*;
Mycobacterium leprae;
Real-Time Polymerase Chain Reaction*;
Reverse Transcriptase Polymerase Chain Reaction;
RNA;
Skin*
- From:Korean Leprosy Bulletin
2015;48(1):17-29
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Mycobacterium leprae, the etiological agent of leprosy, is noncultivable on axenic media, so, the viability of M. leprae for clinical or experimental applications is often unknown. Quantitative reverse transcriptase PCR (RT-PCR) assays were recently introduced as the new tools for M. leprae viability determination. OBJECTIVE: For evaluating of correlation of results of quantitative real-time PCR(16S rRNA/RLEP) & AFB stain of slit skin smear & histopathology & estimating the viability of M. leprae, the author studied the comparison of results of them METHODS: Of 46 samples from 27 patients(MB 24 cases, PB 3 cases), M. leprae 16S rRNA was used as RNA targets, and M. leprae repetitive element (RLEP) DNA was used to determine relative bacterial numbers and the viability by the quantitative real-time PCR. The ratio of 16S rRNA and RLEP as the indicator of viability was calculated. Student t test and linear Pearson correlation were done by SPSS. RESULTS: There was a correlation between between 16S rRNA/RLEP ratio and BI (r=0.369, p=0.012), and was statistically significant between 16S rRNA/RLEP ratio and histopathological positivity of AFB (p=0.011). However there was no correlation between 16S rRNA/RLEP ratio and MI. CONCLUSIONS: Although the correlation between between 16S rRNA/RLEP ratio and BI and the statistically significant between 16S rRNA/RLEP ratio and histopathological positivity of AFB, there was no correlation between 16S rRNA/RLEP ratio and MI. It needs the further evaluation the correlation about that.
