Effects of Immune Aggregates, Hydrocortisone and Angiotensin II Receptor Blocker on Interleukin-6 mRNA Expression in Human Mesangial Cells.
- Author:
Chun Gyoo IHM
1
;
Jae Kyung PARK
;
Tae Won LEE
;
Dae Ryong CHA
Author Information
1. Department of Nephrology, College of Medicine, Kyung Hee University, Korea. cgihm@chollian.net
- Publication Type:Original Article
- Keywords:
Immune aggregates;
Angiotensin II;
Interleukin-6;
AT1 blocker;
Hydrocortisone
- MeSH:
Actins;
Angiotensin II*;
Angiotensins*;
Antigen-Antibody Complex;
Humans*;
Hydrocortisone*;
Immunoglobulin A;
Immunoglobulin G;
Interleukin-6*;
Losartan;
Mesangial Cells*;
Polymerase Chain Reaction;
Receptors, Angiotensin*;
Reverse Transcription;
RNA, Messenger*
- From:Korean Journal of Nephrology
2002;21(2):199-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Various factors regulate interleukin(IL)-6 expression in mesangial cells (MCs). Immune complexes or angiotensin II(AII) are involved in the development of glomerulonephritis(GN). We evaluated the effects of IgG and IgA aggregates or AII on IL-6 mRNA expression in human MCs and the modulation by losartan, an AT1 receptor blocker, or hydrocortisone. METHODS: After 48 hours of culture in the presence of sera, IL-6 mRNA expression was analyzed by reverse transcription and polymerase chain reaction(PCR). RESULTS: Incubation of MCs with IgA or IgG aggregates(100 microgram/mL) as well as AII(10(-7) M) enhanced the ratio of PCR products for IL-6 to beta-actin on densitometric results. The addition of hydrocortisone(0.5 microgrammL) reduced the IgA aggregates-induced IL-6 mRNA expression and losartan(10(-6) M) reduced IgG aggregates- induced IL-6 mRNA expression. CONCLUSION: These results suggest that IgG and IgA aggregates and AII may induce IL-6 expression in GN which can be partially suppressed by hydrocortisone or AT1 receptor blocker.
