Monocyte Chemoattractant Protein-1 Upregulates Fibronectin Secretion by Human Peritoneal Fibroblasts.
- Author:
Mi Ra YU
1
;
Hi Bahl LEE
;
Hun Joo HA
Author Information
1. Hyonam Kidney Laboratory Soon Chun Hyang University, Seoul, Korea. ha@hkl.ac.kr
- Publication Type:Original Article
- Keywords:
Monocyte chemoattractant protein;
Fibronectin;
Mesangial cells;
Peritoneal mesothelial cells;
Peritoneal fibroblasts;
Peritoneal fibrosis
- MeSH:
Animals;
Blotting, Western;
Chemokine CCL2*;
Collagen;
Fibroblasts*;
Fibronectins*;
Fibrosis;
Glucose;
Hand;
Humans*;
Lung;
Mesangial Cells;
Mice;
Monocytes*;
Peritoneal Fibrosis;
Rats;
Transforming Growth Factor beta1
- From:Korean Journal of Nephrology
2002;21(2):259-265
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: High glucose upregulates MCP-1 expression in rat glomerular mesangial cells and in human peritoneal mesothelial cells. However, the role of high glucose-induced MCP-1 on the development and progression of diabetic renal injury and peritoneal injury during peritoneal dialysis(PD) using high glucose PD solutions are not clear. Since MCP-1 was shown to upregulate transforming growth factor-beta1(TGF-beta1) and collagen expression in lung fibroblasts, the present study investigated the effects of MCP-1 on fibronectin secretion by mouse mesangial cells(MMC), human peritoneal mesothelial cells (HPMC), and human peritoneal fibroblasts(HPFB). METHODS: Synchronized cells were stimulated by different concentrations of MCP-1(0.1-100 ng/mL) or TGF-beta1(0.1-10 ng/mL) for 48 hours. Fibronectin protein secreted into the media was analyzed by Western blot analysis. RESULTS: MCP-1 up to 100 ng/mL did not affect fibronectin secretion by MMC. TGF-beta1 10 ng/mL, however, increased fibronectin secretion by MMC 2.8 fold that of control. MCP-1 up to 100 ng/mL did not affect fibronectin secretion by HPMC. But, TGF-beta1 0.1 ng/mL increased fibronectin secretion by HPMC 1.8 fold compared to control. On the other hand, MCP-1 increased fibronectin secretion by HPFB in a dose-dependent manner. MCP-1 at 1-10 ng/mL significantly increased fibronectin when compared to M199 control. 100 ng/mL MCP-1 further increased fibronectin secretion by HPFB compared to 0.1-10 ng/mL MCP-1. CONCLUSION: These results suggest a possible role for MCP-1 in the development and progression of peritoneal fibrosis and support the view that in addition to recruiting inflammatory cells MCP-1 may play a role in tissue fibrosis in other organs.
