The application of TD (Touch-Down) PCR in Diagnosis of leprosy and Detection of MDT-resistant M. leprae.
- Author:
Se Kon KIM
1
;
Seong Beom LEE
;
Tae Jin KANG
;
Gue Tae CHAE
Author Information
1. Institute of Hansen's Disease, Korea.
- Publication Type:Original Article
- Keywords:
Leprosy;
RLEP repetitive sequence;
TD (Touch-Down) PCR
- MeSH:
Diagnosis*;
DNA;
Leprosy*;
Polymerase Chain Reaction*;
Repetitive Sequences, Nucleic Acid
- From:Korean Leprosy Bulletin
2001;34(2):13-21
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
There are several methods for diagnosis of leprosy, including AFB stain, the measurement of PGL-1 (phenolic glycolipid - 1) antigen titer, and DNA-PCR. In this study, we have used the DNA-PCR amplifying the RLEP repetitive sequence. Our result showed that the RLEP primer offered the more sensitive detection and identification of M. leprae DNA in clinical specimens, compared with the other primer, for example, 18-kDa antigen gene. To screen the resistant M. leprae strain of MDT (Multi-Drug Therapy), we have used the TD (Touch-Down) PCR. We arranged and amplified sequences of the genes, folP, rpoB, gyr, 23S rRNA, in M. leprae involved in MDT-resistance, and could obtain the PCR product each gene, simultaneously. This method, based on annealing temperature, was useful to the detection for diagnosis and the screen of MDT-resistant strain of M. leprae, rapidly. Thus, we suggest that the RLEP primer and TD-PCR method are effective in assessing the diagnosis of leprosy and the identification of drug-resistant M. leprae.
