The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells.
10.5395/JKACD.2009.34.4.356
- Author:
Young Eun LEE
1
;
Eui Seong KIM
;
Jin KIM
;
Seung Hoon HAN
;
Seung Jong LEE
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Korea. sjlee@yuhs.ac
- Publication Type:Original Article
- Keywords:
periodontal ligament cell;
MTT;
WST-1;
viability;
pressure;
cryo-preservation
- MeSH:
Anesthesia;
Animals;
Baths;
Dimethyl Sulfoxide;
Eosine Yellowish-(YS);
Female;
Freezing;
Humans;
Molar;
Periodontal Ligament;
Rats;
Tiletamine;
Tooth
- From:Journal of Korean Academy of Conservative Dentistry
2009;34(4):356-363
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4degrees C for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37degrees C water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.
