Effects of 7-Nitroindazole on Brain Cell Membrane Function and Energy Metabolism during Transient Global Cerebral Hypoxia-Ischemia and Reoxygenation-Reperfusion in Newborn Piglets.
- Author:
Chang Won CHOI
1
;
Jong Hee HWANG
;
Yun Sil CHANG
;
Won Soon PARK
Author Information
1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. wspark@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Hypoxia-ischemia;
Brain;
7-Nitroindazole;
Reperfusion injury;
Metabolism;
Animals;
Newborn
- MeSH:
Adenosine Triphosphate;
Animals;
Brain*;
Carotid Artery, Common;
Cell Membrane*;
Energy Metabolism*;
Humans;
Hypoxia-Ischemia, Brain*;
Infant, Newborn*;
Metabolism;
Neuroprotective Agents;
Nitric Oxide Synthase Type I;
Oxygen;
Polymerase Chain Reaction;
Reperfusion Injury;
Respiration;
Ventilation
- From:Korean Journal of Pediatrics
2004;47(2):204-209
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Our study was undertaken to discover whether a selective neuronal nitric oxide synthase inhibitor, 7-nitroindazole, influences brain cell membrane function and energy metabolism during and after transient global hypoxia-ischemia(HI) in newborn piglets. METHODS: Cerebral HI was induced by temporary complete occlusion of bilateral common carotid arteries and simultaneous breathing with 8% oxygen for 30 minutes, followed by release of carotid occlusion and normoxic ventilation for one hour(reoxygenation-reperfusion, RR). 7-Nitroindazole(50 mg/kg) or vehicle was administered intraperitoneally just before the induction of HI or RR. Brain cortex was harvested for the biochemical analysis at the end of HI or RR. RESULTS: The level of conjugated dienes significantly increased and the activity of Na+, K+-ATPase significantly decreased during HI, and they did not recover during RR. The levels of ATP and phosphocreatine(PCr) significantly decreased during HI, and recovered during RR. 7-Nitroindazole did not influence significantly the level of conjugated dienes, the activity of Na+, K+-ATPase, and the levels of ATP and PCr during HI and RR. CONCLUSION: 7-nitroindazole did not demonstrate a neuroprotective effect in our piglet model of transient global cerebral HI and one hour of RR.
