The Cytotoxicity of Poly - L - lysine in Different Concentration to Preadipocytes Harvested from Living Rats.
- Author:
Ho KWON
1
;
Yoon SEOK
;
Jong Won RHIE
;
Gyeol YOO
;
Jin Soo LIM
;
Sang Hoon CHUNG
;
Sang Tae AHN
Author Information
1. Department of Plastic Surgery, College of Medicine, The Catholic University of Korea.
- Publication Type:Original Article
- Keywords:
Preadipocyte;
Poly-L-lysine;
Cytotoxicity
- MeSH:
Adipocytes;
Animals;
Culture Media;
Lysine*;
Rats*
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2000;27(6):683-686
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Authors are trying to prove the fact that Poly-L-lysine (PLL) might be mixed with alginate to enhance cell-to- matrix adhesion. Before that experiment, the proportion of preadipocytes in cells obtained from rat epididymal fat was detected, and PLL cytotoxicity on preadipocytes was measured. All cells harvested after third passage of culture were differentiated into adipocytes, and there was no decrease in the proliferation of preadipocytes in the culture media under the PLL concentration of 5 microgram/m4. These results suggest that all cells harvested from rat epididymal fat after 3rd passage of culture were preadipocytes and PLL has no cytotoxicity to preadipocytes of rats under the concentration of 5 microgram/mP.
