Significance of Preoperative Tissue Levels of Vascular-endothelial Cadherin, Liver-intestine Cadherin and Vascular Endothelial Growth Factor in Gastric Cancer.
10.4166/kjg.2012.60.4.229
- Author:
Kum Hei RYU
1
;
Ki Nam SHIM
;
Sung Ae JUNG
;
Kwon YOO
;
Yang Hee JOO
;
Joo Ho LEE
Author Information
1. Center for Cancer Prevention and Detection, National Cancer Center, Goyang, Korea.
- Publication Type:Original Article
- Keywords:
Cadherin;
Vascular endothelial growth factor;
Stomach neoplasms
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Antigens, CD/genetics/*metabolism;
Cadherins/genetics/*metabolism;
Female;
Gastroscopy;
Humans;
Immunohistochemistry;
Lymphatic Metastasis;
Male;
Middle Aged;
Retrospective Studies;
Stomach Neoplasms/*metabolism/pathology/surgery;
Vascular Endothelial Growth Factor A/genetics/*metabolism
- From:The Korean Journal of Gastroenterology
2012;60(4):229-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The aims of this study were to examine the expressions of endothelium specific VE-cadherin, intestine specific LI-cadherin, and vascular endothelial growth factor (VEGF), and to determine their relationships with the clinicopathological parameters of gastric cancer. METHODS: A total 47 patients with gastric cancer who underwent surgery were enrolled. Endoscopic biopsies were obtained from the cancer and normal mucosa, respectively. Using semiquantitative RT-PCR, the mRNA expression levels of VE-cadherin, LI-cadherin and VEGF were measured by tumor/normal (T/N) ratios. The protein expressions of VE-cadherin, LI-cadherin and VEGF were examined by Western blot and immunohistochemical stain in surgically resected tissues. The clinicopathological variables were reviewed and analyzed, retrospectively. RESULTS: Twenty two cases (46.8%) of VE-cadherin, 25 cases (53.2%) of LI-cadherin and 27 cases (51.1%) of VEGF mRNA expressions were overexpressed in gastric cancer compared to normal tissue. There was a tendency for T/N ratio of VE-cadherin mRNA to correlate with the lymphatic invasion (p=0.07) and the lymph node metastasis (p=0.099) in advanced gastric cancer. The T/N ratio of LI-cadherin mRNA showed significant association with distant metastasis (p=0.031) and lymphatic invasion especially in advanced gastric cancer (p=0.023). There was a tendency for the T/N ratio of VEGF mRNA to correlate with the distant metastasis (p=0.073) in advanced gastric cancer. CONCLUSIONS: As increased mRNA expression of LI-cadherin was associated with distant metastasis and lymphatic invasion especially in the biopsy specimen of advanced gastric cancer before surgery, it may provide useful preoperative information on tumor aggressiveness.
