Effect of β-carotene on Cell Growth Inhibition of KB Human Oral Cancer Cells.
10.11620/IJOB.2016.41.3.105
- Author:
Sung Su YANG
1
;
Su Gwan KIM
;
Byung Sun PARK
;
Dae San GO
;
Sun Kyoung YU
;
Chun Sung KIM
;
Jeongsun KIM
;
Do Kyung KIM
Author Information
1. Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea. kdk@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
β-carotene;
cell death;
apoptosis;
oral cancer cells
- MeSH:
Apoptosis;
Caspase 3;
Cell Death;
Cell Proliferation;
Cucurbita;
Daucus carota;
Drug Discovery;
Humans*;
Ipomoea batatas;
KB Cells;
Mouth Neoplasms*
- From:International Journal of Oral Biology
2016;41(3):105-111
- CountryRepublic of Korea
- Language:English
-
Abstract:
β-carotene is present in carrots, pumpkins, and sweet potatoes. It suppresses many types of cancers by regulating cellular proliferation and apoptosis through a variety of mechanisms. However, the effects of β -carotene on oral cancer cells have not been clearly established. The main goal of this study was to investigate the effects of β-carotene on cell growth and apoptosis in oral cancer cells. Our results demonstrate that treatment with β-carotene induced inhibition of cell growth, and that the effect was dependent on β-carotene treatment time and concentration in KB cells. Furthermore, treatment with β-carotene induced nuclear condensation and fragmentation in KB cells. β-carotene promoted proteolytic cleavage of procaspase-3, -7, -8 and -9 with associated increases in the concentration of cleaved caspase-3, -7, -8 and -9. In addition, the level of cleaved PARP was increased by β-carotene treatment in KB cells. These results suggest that β-carotene can suppress cell growth and induce apoptosis in KB human oral cancer cells, and that it may have potential usefulness in anti-cancer drug discovery efforts.
