Participation of IL-1β in temporomandibular nociception in rats with CFA-induced inflammation.
10.11620/IJOB.2016.41.3.125
- Author:
Jin Sook JU
1
;
Seung Ho CHOI
;
Hye Jin KIM
;
Jo Young SON
;
Dong Kuk AHN
Author Information
1. Department of Oral Physiology, School of Dentistry, Kyungpook National University, Daegu, Korea. dkahn@knu.ac.kr
- Publication Type:Original Article
- Keywords:
IL-1β;
TMJ;
nociception;
CFA;
formalin behavior test
- MeSH:
Animals;
Formaldehyde;
Humans;
Hyperalgesia;
Inflammation*;
Injections, Intra-Articular;
Interleukin-1;
Male;
Models, Animal;
Nociception*;
Rats*;
Rats, Sprague-Dawley;
Temporomandibular Joint
- From:International Journal of Oral Biology
2016;41(3):125-131
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of the present study was to develop an animal model for evaluation of temporomandibular (TMJ) nociception under TMJ inflammation. We also investigated the participation of IL-1β in inflammation-induced TMJ nociception. Experiments were carried out using male Sprague-Dawley rats. Intra-articular injection of 3% formalin was administered to evaluate hyperalgesia 3 days after CFA injection. Intra-articular injection of 3% formalin did not produce nociceptive behavior in normal rats. Although intra-articular injection of 3 doses of CFA produced TMJ inflammation, only 1:3 diluted CFA produced hyperalgesia when formalin was injected intra-articularly 3 days after CFA injection. Co-administration of IL-1 receptor inhibitor with formalin into the TMJ cavity 3 days after CFA injection was performed. Co-administration of IL-1 receptor inhibitor significantly inhibited formalin-induced hyperalgesia in rats with CFA-induced TMJ inflammation. These results suggested that intra-articular injection of formalin produced hyperalgesia under chronic TMJ inflammation. Moreover, IL-1β plays an important role in TMJ hyperalgesia under chronic inflammation and blockade of IL-1β is a potential therapeutic target for inflammatory TMJ pain.
