Effect of serum-derived albumin scaffold and canine adipose tissue-derived mesenchymal stem cells on osteogenesis in canine segmental bone defect model.
10.4142/jvs.2015.16.4.397
- Author:
Daeyoung YOON
1
;
Byung Jae KANG
;
Yongsun KIM
;
Seung Hoon LEE
;
Daeun RHEW
;
Wan Hee KIM
;
Oh Kyeong KWEON
Author Information
1. Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. ohkweon@snu.ac.kr
- Publication Type:Original Article
- Keywords:
adipose tissue-derived mesenchymal stem cells;
bone defect;
serum-derived albumin scaffold
- MeSH:
Allografts;
Animals;
Dogs;
Mesenchymal Stromal Cells*;
Osteogenesis*;
Stem Cells;
Transplants
- From:Journal of Veterinary Science
2015;16(4):397-404
- CountryRepublic of Korea
- Language:English
-
Abstract:
Composite biological and synthetic grafts with progenitor cells offer an alternative approach to auto- or allografts for fracture repair. This study was conducted to evaluate osteogenesis of autologous serum-derived albumin (ASA) scaffolds seeded with canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) in a canine segmental bone defect model. ASA scaffold was prepared with canine serum using cross-linking and freeze-drying procedures. Beta-tricalcium phosphate (beta-TCP) was mixed at the cross-linking stage. Ad-MSCs were seeded into the scaffold and incubated for one day before implantation. After 16 weeks, the grafts were harvested for histological analysis. The dogs were divided into five groups: control, ASA scaffolds with and without Ad-MSCs, and ASA scaffolds including beta-TCP with and without Ad-MSCs. ASA scaffolds with Ad-MSCs had a significantly larger area of increased opacity at the proximal and distal host cortex-implant interfaces in radiographs 16 weeks after implantation compared to the groups with beta-TCP (p < 0.05). Histomorphometric analysis showed that ASA scaffolds with Ad-MSCs had significantly greater new bone formation than other groups (p < 0.05). These results suggest that Ad-MSCs seeded into ASA scaffolds enhanced osteogenesis in the bone defect model, but that beta-TCP in the ASA scaffold might prevent penetration of the cells required for bone healing.
