Effects of Culture Condition on Secretion of Phospholipase from Candida albicans.
- Author:
Woon Seob SHIN
1
;
Kyoung Ho LEE
;
Joo Young PARK
;
Choon Myung KOH
Author Information
1. Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Korea.
- Publication Type:Original Article
- Keywords:
Candida;
albicans;
Phospholipase;
Production
- MeSH:
Agar;
Candida albicans*;
Candida*;
Citric Acid;
Deoxycholic Acid;
Glucose;
Hydrogen-Ion Concentration;
Immunocompromised Host;
Lecithins;
Phospholipases*;
Soaps;
Virulence;
Yeasts
- From:Korean Journal of Medical Mycology
1997;2(2):123-128
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathogenesis of Candida infections. OBJECTIVE: In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans. METHODS: C. albicans ATCC 10231 was cultivated in various media at 37 degrees C for 3 days. Phospholipase activity was measured by fatty acid soap precipitation in plate containing 0.04% lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar. RESULTS: Phospholipase was highly induced when C. albicans was cultivated in broth medium (containing glucose 2%, albumin 0.2% and Fe++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate. CONCLUSION: Highly induction of secreted phospholipase by albumin from C albicans may be play an important role in tissue invasion in the pathogenesis of C. albicans.
