A Study in the Effects of Epidermal Growth Factor and Transforming Growth Factors-alpha on the Growth of Human Transitional Cell Carcinoma Cell Lines.
- Author:
Ki Yong SHIN
1
;
Sun Jin KIM
;
Weon Ho CHOI
;
Do Yeon CHOI
;
Hae Young PARK
;
Tchun Yong LEE
;
Gu KONG
;
Young Nam WOO
;
Dong Han KIM
Author Information
1. Department of Urology, School of Medicine, Han Yang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Epidermal growth factor;
Transforming growth factors;
Human transitional cell carcinoma cell lines
- MeSH:
Carcinoma, Transitional Cell*;
Cell Line*;
Culture Media, Serum-Free;
Epidermal Growth Factor*;
Humans*;
Recurrence;
Transforming Growth Factor alpha;
Transforming Growth Factors;
Urinary Bladder;
Urothelium
- From:Korean Journal of Urology
1998;39(1):45-50
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We evaluated the effects of epidermal growth factor(EGF) and transforming growth factor-alpha (TGF-alpha) on the growth of human transitional cell carcinoma cell lines. MATERIALS AND METHODS: T-24, KK47 and KU-1 cell lines established from human transitional cell carcinoma tissues were used. We cultured the cell lines in serum-free media for 48 hours and then administered EGF and TGF-alpha in the concentrations of 0.1ng/ml, 1ng/ml, 10ng/ml and 100ng/m1 respectively. We analysed the effects by MTT assay at 144 hours. RESULTS: There were statistically significant stimulatory effects on the growth of three cell lines in all cultures. Enhanced growth was also observed in the culture with administration of EGF and TGF-alpha simultaneously. None of stimulatory effects were dose-related. CONCLUSIONS: We should take into consideration of possible role of EGF which are excreted in urine in very high concentration in a biologically active form and TGF-alpha of which urinary level increases with various type of bladder injuries in occurrence, progression and recurrence of transitional cell carcinoma of urothelium.
