Dual-Modal Nanoprobes for Imaging of Mesenchymal Stem Cell Transplant by MRI and Fluorescence Imaging.
10.3348/kjr.2009.10.6.613
- Author:
Chang Kyu SUNG
1
;
Kyung Ah HONG
;
Shunmei LIN
;
Yuwon LEE
;
Jinmyung CHA
;
Jin Kyu LEE
;
Cheol Pyo HONG
;
Bong Soo HAN
;
Sung Il JUNG
;
Seung Hyup KIM
;
Kang Sup YOON
Author Information
1. Department of Radiology, Seoul Metropolitan Boramae Medical Center, Seoul National University College of Medicine, Seoul 156-707, Korea. sckmd@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Stem cells;
Nanoparticles;
Magnetic resonance (MR);
Optical imaging, contrast agent
- MeSH:
Animals;
Biocompatible Materials;
Cells, Cultured;
Cobalt;
Feasibility Studies;
Ferric Compounds;
Humans;
Magnetic Resonance Imaging/*methods;
*Mesenchymal Stem Cells;
Mice;
Mice, Nude;
Microscopy, Confocal;
Microscopy, Electron;
Nanoparticles/*chemistry;
Phantoms, Imaging;
Polyethylene Glycols;
Rats;
Rhodamines;
Silicon Dioxide;
Staining and Labeling/methods
- From:Korean Journal of Radiology
2009;10(6):613-622
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To determine the feasibility of labeling human mesenchymal stem cells (hMSCs) with bifunctional nanoparticles and assessing their potential as imaging probes in the monitoring of hMSC transplantation. MATERIALS AND METHODS: The T1 and T2 relaxivities of the nanoparticles (MNP@SiO2[RITC]-PEG) were measured at 1.5T and 3T magnetic resonance scanner. Using hMSCs and the nanoparticles, labeling efficiency, toxicity, and proliferation were assessed. Confocal laser scanning microscopy and transmission electron microscopy were used to specify the intracellular localization of the endocytosed iron nanoparticles. We also observed in vitro and in vivo visualization of the labeled hMSCs with a 3T MR scanner and optical imaging. RESULTS: MNP@SiO2(RITC)-PEG showed both superparamagnetic and fluorescent properties. The r1 and r2 relaxivity values of the MNP@SiO2(RITC)-PEG were 0.33 and 398 mM-1 s-1 at 1.5T, respectively, and 0.29 and 453 mM-1 s-1 at 3T, respectively. The effective internalization of MNP@SiO2(RITC)-PEG into hMSCs was observed by confocal laser scanning fluorescence microscopy. The transmission electron microscopy images showed that MNP@SiO2(RITC)-PEG was internalized into the cells and mainly resided in the cytoplasm. The viability and proliferation of MNP@SiO2(RITC)-PEG-labeled hMSCs were not significantly different from the control cells. MNP@SiO2(RITC)-PEG-labeled hMSCs were observed in vitro and in vivo with optical and MR imaging. CONCLUSION: MNP@SiO2(RITC)-PEG can be a useful contrast agent for stem cell imaging, which is suitable for a bimodal detection by MRI and optical imaging.
