Lactoferrin as a gene delivery vehicle to hepatocytes.
- Author:
Sang Taek OH
1
;
Jeong Keun RIH
;
Heung Sun KWON
;
Deog Su HWANG
;
Sun Young KIM
;
Jeong Bin YIM
Author Information
1. SEOUL NATL UNIV, INST MOL BIOL & GENET, SEOUL 151742, SOUTH KOREA.
- Publication Type:Original Article
- Keywords:
lactoferrin;
poly-L-lysine;
gene delivery
- MeSH:
Animals;
Cats;
Chromatography;
Dialysis;
Fibroblasts;
Filtration;
Genes, Reporter;
Genes, vif*;
Hepatocytes*;
Humans;
Lac Operon;
Lactoferrin*;
Liver;
Mice;
Polylysine;
Transfection
- From:Experimental & Molecular Medicine
1997;29(2):111-116
- CountryRepublic of Korea
- Language:English
-
Abstract:
Using lactoferrin as the specific ligand, we developed a simplified method for preparation of molecular conjugate for gene delivery. Replacement of column chromatography and dialysis by one step centrifugal filtration (Centricon, cut off size : 30,000), resulted in the rapid purification of bovine lactoferrin/polylysine (bLf/pL) and human lactoferrin/polylysine (hLf/pL) conjugates and easy separation of unconjugated polylysine. The Lf/pL conjugates prepared by this method efficiently transferred the reporter genes, CAT and LacZ gene, to HeLa and hepatic cells. The bLf/pL and hLf/pL conjugates could transfer the reporter genes to various hepatocytes including primary mouse hepatocyte, Hepa 1-6, SK-Hep1 and Chang liver, but not to NIH 3T3 mouse fibroblast cells, indicating that the Lf/pL conjugates conferred hepatocyte-specific gene transfer. The bLf/pL and hLf/pL conjugates prepared in the present study exhibited higher transfection efficiencies for mouse and human hepatocytes than the commercially available transferrin/polylysine (Tf/pL) conjugate.
