Species specificity of a novel factor which augments the expression of MHC class I antigens on tumor cell lines.
- Author:
Niti PURI
1
;
Aparna RAVAL
;
Rajiv K SAXENA
Author Information
1. JAWAHARLAL NEHRU UNIV, SCH LIFE SCI, NEW DELHI 110067, INDIA.
- Publication Type:Original Article
- Keywords:
major histocompatibility complex;
lymphokines;
lymphocytes;
interferon gamma
- MeSH:
Animals;
Cell Line;
Cell Line, Tumor*;
Hand;
Histocompatibility Antigens Class I*;
Humans;
Interferons;
Lymphocyte Culture Test, Mixed;
Lymphocytes;
Lymphokines;
Major Histocompatibility Complex;
Mice;
Mitogens;
Species Specificity*;
Spleen
- From:Experimental & Molecular Medicine
1997;29(2):129-132
- CountryRepublic of Korea
- Language:English
-
Abstract:
A novel factor which augments the expression of major histocompatibility complex I (MHC augmenting factor or MHC-AF) antigens on tumor cell lines, has been isolated from the culture supernatants of human peripheral blood mononuclear cells activated by concanavalin-A. A mouse equivalent of this factor has also been isolated from the culture supernatants of mouse spleen cells activated by mitogens or in a mixed lymphocyte reaction. Mouse MHC-AF enhances the expression of class I MHC antigens on murine tumor cell lines (EL-4 and BW5147) but not on human tumor cell lines (K562 and HR-7). Human MHC-AF on the other hand enhances the MHC I expression on both human as well as murine cell lines. Interferon gamma (IFN-gamma), a cytokine also known to enhance the expression of MHC I antigens, acts in a highly species specific manner with mouse IFN-gamma augmenting the MHC I on murine tumor cell lines and human IFN-gamma augmenting the MHC I on human tumor cell lines only. These results indicate important differences in the cross species biological activities of MHC-AF and IFN-gamma, and provide additional evidence for MHC-AF being distinct from IFN-gamma.
