The Matrix metalloproteinase-2 & -9, Tissue inhibitor of metalloproteinase-1 & -2 mRNA Expression in Invasive Cervical Cancer.
- Author:
Hye Sung MOON
1
;
Eun Ah CHOI
;
Min Young YOO
;
Hye Won CHUNG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
invasive cervical cancer;
MMP-2 & -9;
TIMP-1 & -2 mRNA
- MeSH:
Basement Membrane;
Biology;
Cervix Uteri;
DNA, Complementary;
Female;
Humans;
Matrix Metalloproteinase 2*;
Matrix Metalloproteinases;
Neoplasm Metastasis;
RNA;
RNA, Messenger*;
Tissue Inhibitor of Metalloproteinase-1*;
Tissue Inhibitor of Metalloproteinase-2;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2001;44(3):532-539
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Invasive cancer cells penetrate the extracellular matrix(ECM), including basement membrane during the metastatic cascades. Matrix metalloproteinases(MMPs) play a critical role in tumor invasion and metastasis and their activities are regulated by specific tissue inhibitors of metalloproteinase(TIMPs). Aberrant ECM degradation in tumor biology is attributed to an imbalance in local MMP and TIMP activity, resulting in the overexpression or enhanced activation of MMPs or reduced TIMP expression. The aim of this study was to compare the MMP-2 & -9, TIMP-1 & -2 mRNA expression in cervical cancer with those in normal cervix and to investigate that their expression is related to cancer stages and other prognostic factors. METHODS: The normal cervix and cervical cancer tissues were obtained from healthy women(n=14), and the patients with cervical cancer(n=31), respectively. Total RNA was extracted and reverse transcribed into cDNA. The expression of MMP-2 & -9, TIMP-1 & -2 mRNA was examined by quantative competitive PCR(QC PCR) and each results were analyzed by t-test and univariate analysis. RESULTS: The expression of MMP-2, TIMP-1 mRNA in cervical cancer was higher than that in nomal cervix(p<0.05). The TIMP-2 mRNA expression was elevated in cervical cancer while that was not shown in normal cervix. The MMP-9 mRNA expression was not statistically different between normal cervix and cervical cancer(p>0.05). CONCLUSIONS: These results suggested that increased MMP-2, TIMP-1 & -2 mRNA expression is an early event during malignant transformation of cervical cancer.
