Effects of DA-9701, a Novel Prokinetic Agent, on Phosphorylated Extracellular Signal-Regulated Kinase Expression in the Dorsal Root Ganglion and Spinal Cord Induced by Colorectal Distension in Rats.
- Author:
Sang Pyo LEE
1
;
Kang Nyeong LEE
;
Oh Young LEE
;
Hang Lak LEE
;
Dae Won JUN
;
Byung Chul YOON
;
Ho Soon CHOI
;
Se Jin HWANG
;
Seo Eun LEE
Author Information
1. Department of Internal Medicine, Digestive Disease Centre, Konkuk University School of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
DA-9701;
Gastrointestinal diseases;
Visceral hypersensitivity;
Colorectal distension;
Phosphorylated extracellular signal-regulated kinase
- MeSH:
Analgesics/*pharmacology;
Animals;
Colon;
Dilatation, Pathologic/physiopathology;
Down-Regulation;
Extracellular Signal-Regulated MAP Kinases/drug effects/*metabolism;
Ganglia, Spinal/drug effects/*metabolism;
Male;
Phytotherapy/methods;
Plant Preparations/*pharmacology;
Proto-Oncogene Proteins c-fos/metabolism;
Rats;
Rats, Sprague-Dawley;
Rectum;
Spinal Cord/drug effects/*metabolism;
Substance P/metabolism;
Visceral Pain/prevention & control
- From:Gut and Liver
2014;8(2):140-147
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: DA-9701, a standardized extract of Pharbitis Semen and Corydalis Tuber, is a new prokinetic agent that exhibits an analgesic effect on the abdomen. We investigated whether DA-9701 affects visceral pain induced by colorectal distension (CRD) in rats. METHODS: A total of 21 rats were divided into three groups: group A (no CRD+no drug), group B (CRD+no drug), and group C (CRD+DA-9701). Expression of pain-related factors, substance P (SP), c-fos, and phosphorylated extracellular signal-regulated kinase (p-ERK) in the dorsal root ganglion (DRG) and spinal cord was determined by immunohistochemical staining and Western blotting. RESULTS: The proportions of neurons in the DRG and spinal cord expressing SP, c-fos, and p-ERK were higher in group B than in group A. In the group C, the proportion of neurons in the DRG and spinal cord expressing p-ERK was lower than that in group B. Western blot results for p-ERK in the spinal cord indicated a higher level of expression in group B than in group A and a lower level of expression in group C than in group B. CONCLUSIONS: DA-9701 may decrease visceral pain via the downregulation of p-ERK in the DRG and spinal cord.
