Identifying Genetic Susceptibility to Sensitization to Cephalosporins in Health Care Workers.
10.3346/jkms.2012.27.11.1292
- Author:
Young Hee NAM
1
;
Jeong Eun KIM
;
Seung Hyun KIM
;
Hyun Jung JIN
;
Eui Kyung HWANG
;
Yoo Seob SHIN
;
Young Min YE
;
Hae Sim PARK
Author Information
1. Department of Allergy & Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@ajou.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cephalosporins;
FcepsilonR1beta Gene;
IgE;
IgG;
Single-Nucleotide Polymorphism
- MeSH:
Adult;
Alleles;
Anti-Bacterial Agents/analysis/*immunology;
Cephalosporins/analysis/*immunology;
Enzyme-Linked Immunosorbent Assay;
Female;
Genetic Predisposition to Disease;
Health Personnel;
Humans;
Hypersensitivity/*diagnosis/epidemiology;
Immunoglobulin E/blood;
Immunoglobulin G/blood;
Male;
Occupational Diseases/*chemically induced/epidemiology;
Occupational Exposure;
Odds Ratio;
Questionnaires;
Receptors, IgE/genetics;
Skin Tests;
Young Adult
- From:Journal of Korean Medical Science
2012;27(11):1292-1299
- CountryRepublic of Korea
- Language:English
-
Abstract:
Exposure to cephalosporins could cause occupational allergic diseases in health care workers (HCWs). We evaluated the prevalence of serum specific IgE and IgG antibodies to cephalosporin-human serum albumin (HSA) conjugate and to identify potential genetic risk factors associated with sensitization to cephalosporins in exposed HCWs. The study population consisted of 153 HCWs who had been exposed to antibiotics in a single university hospital and 86 unexposed healthy controls. A questionnaire survey of work-related symptoms (WRS) was administered. A skin-prick test (SPT) was performed, and serum-specific IgE and IgG antibodies to 3 commonly prescribed cephalosporins were measured by ELISA. Four single-nucleotide polymorphisms of the candidate genes related to IgE sensitization were genotyped. The prevalence of WRS to cephalosporins was 2.6%. The prevalence rates of serum-specific IgE and IgG antibodies to cephalosporins were 20.3% and 14.7%, respectively. The FcepsilonR1beta-109T > C polymorphism was significantly associated with IgE sensitization to cephalosporins in HCWs (P = 0.036, OR = 3.553; CI, 1.324-9.532). The in vitro functional assay demonstrated that the T allele of FcepsilonR1beta-109T had greater promoter activity than did the C allele (P < 0.001). The FcepsilonR1beta-109T > C polymorphism may be a potential genetic risk factor for increased IgE sensitization to cephalosporins.
