Human Amniotic Fluid Stem Cell-derived Muscle Progenitor Cell Therapy for Stress Urinary Incontinence.
10.3346/jkms.2012.27.11.1300
- Author:
So Young CHUN
1
;
Deok Hyun CHO
;
Seon Yeong CHAE
;
Kyung Hee CHOI
;
Hyun Ju LIM
;
Ghil Suk YOON
;
Bum Soo KIM
;
Bup Wan KIM
;
James J YOO
;
Tae Gyun KWON
Author Information
1. Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Korea. tgkwon@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Amniotic Fluid;
Stem Cells;
Cell Differentiation;
Urinary Sphincter;
Regeneration;
Urinary Incontinence
- MeSH:
Amniotic Fluid/*cytology;
Animals;
Biological Markers/metabolism;
Cell Differentiation;
Cell Lineage;
Cell Transformation, Neoplastic;
Cells, Cultured;
Coculture Techniques;
Female;
Gene Expression Regulation;
Humans;
Immunohistochemistry;
Mice;
Mice, Inbred ICR;
Regeneration;
*Stem Cell Transplantation;
Stem Cells/*cytology/metabolism;
Urethra/physiology;
Urinary Incontinence, Stress/pathology/*therapy;
Urodynamics
- From:Journal of Korean Medical Science
2012;27(11):1300-1307
- CountryRepublic of Korea
- Language:English
-
Abstract:
The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter.
