Effect of Decursin on the Expression of beta-Catenin and Matrix Metalloproteinase-7 in Prostate Cancer Cell Lines.
- Author:
Ik jun CHOI
1
;
Kweonsik MIN
;
Sangtaek OH
;
Gyu yong SONG
;
Dongil KANG
Author Information
- Publication Type:Original Article
- Keywords: Decursin; Beta-catenin; Matrix metalloproteinase; Prostate
- MeSH: Apoptosis; Benzopyrans; beta Catenin; Blotting, Western; Butyrates; Cell Death; Cell Line; Cell Survival; Humans; Matrix Metalloproteinase 7; Prostate; Prostatic Neoplasms; Real-Time Polymerase Chain Reaction; RNA, Messenger
- From:Korean Journal of Urology 2009;50(1):81-88
- CountryRepublic of Korea
- Language:Korean
- Abstract: PURPOSE: Alterations in the Wnt/beta-catenin pathway are associated with the development and progression of human prostate cancer. Decursin can attenuate the Wnt/beta-catenin pathway. We investigated the relationship between the Wnt/beta-catenin pathway and decursin in prostate cancer cells. MATERIALS AND METHODS: PC-3 and LNCaP cell lines were used. Cell viability was measured with methyl-thiazole tetrazolium bromide (MTT) assays, and cell apoptosis analysis was performed by FACScan. The amount of beta-catenin protein after treatment with decursin was measured by Western blot analysis. Expression of MMP-7 mRNA was detected by real-time polymerase chain reaction (RT-PCR). RESULTS: Death and apoptosis were increased after treatment with decursin 0.5-100 micrometer in PC-3 and LNCaP cells. This was revealed dose and time-dependent increase of cancer cell death on 24, 48 and 72 hours. FACScan showed an increment of apoptosis on 24, 48 hours. Expression of intracellular beta-catenin protein was decreased dose-dependently in both of prostate cancer cell lines. Decursin reduced MMP-7 mRNA expression on 6, 12, 24, 48 hours dose-dependently. CONCLUSIONS: Decursin affects the viability of prostate cancer cells. Increased cancer cell death was associated with increased apoptosis. This study suggests that decursin may play a role in the treatment of prostate cancer.
