Detection Rates of Bacteria in Chronic Otitis Media with Effusion in Children.
10.3346/jkms.2004.19.5.735
- Author:
Chul Won PARK
1
;
Jang Hee HAN
;
Jin Hyeok JEONG
;
Seok Hyun CHO
;
Mi Jung KANG
;
Kyung TAE
;
Seung Hwan LEE
Author Information
1. Department of Otolaryngology, College of Medicine, Hanyang University, Seoul, Korea. shleemd@hanyang.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Otitis Media with Effusion;
Bacteria;
Polymerase Chain Reaction;
Hemphilus influenzae;
Streptococcus pneumoniae;
Moraxella (Branhamella) catarrhalis
- MeSH:
Child;
Child, Preschool;
Chronic Disease;
DNA, Bacterial/analysis;
Haemophilus Infections/*diagnosis;
Haemophilus influenzae/genetics/*isolation & purification;
Humans;
Infant;
Moraxella (Branhamella) catarrhalis/genetics/isolation & purification;
Moraxellaceae Infections/diagnosis;
Otitis Media with Effusion/*diagnosis/*microbiology;
Polymerase Chain Reaction;
Research Support, Non-U.S. Gov't;
Streptococcal Infections/diagnosis;
Streptococcus pneumoniae/genetics/isolation & purification
- From:Journal of Korean Medical Science
2004;19(5):735-738
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to investigate polymerase chain reaction-based detection of bacterial DNA in middle ear fluid and assess the correlation between the PCR-positive rate with several factors associated with middle ear effusion. The purpose was to gain a further understanding of bacterial infection as a major cause of otitis media with effusion. Of the 278 specimens of middle ear fluid, 39 (14%) tested positive by ordinary culture. The overall detection rate of bacterial DNA using the PCR method was 36.7% for middle ear effusion, and bacterial DNA detection rates of Hemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis in the middle ear effusion were 29.1%, 4.7% and 10.8%, respectively. The bacterial DNA detection rate was higher in ears with a history of acute otitis media than those without the history. High detection rates were observed in patients younger than 48 months who have had a higher tendency to present with acute otitis media. We concluded that PCR is a more sensitive method for the detection of bacteria in middle ear effusion than ordinary culture, and acute otitis media is a major contributor to the pathogenesis of otitis media with effusion.
